Our Genome Editing research utilizes CRISPR-Cas9 technology to modify genetic material in various models, aiming to understand disease mechanisms and develop targeted therapies.
The Art of Genomics: Creating Knockout Mice at Our Institute
Welcome to The Art of Genomics, where cutting-edge science meets precision and innovation at our Institute. Here, we embark on a journey to decode the mysteries of life by creating knockout (KO) mice—a revolutionary model for understanding gene function, disease mechanisms, and therapeutic interventions. This is how we, at the forefront of genomic research, make it happen.
Step 1: Mating – Where Life Begins
At our Institute, we believe that every discovery begins with nature. Female mice are carefully superovulated using precise doses of PMSG and hCG, setting the stage for their pairing with fertile males. The presence of a vaginal plug the next morning is our first sign of success, indicating fertilization has occurred.
The fertilized oocytes, still nestled within the oviducts, are then gently harvested under a high-powered microscope by our expert team. These oocytes are the canvas on which we paint the future of genomics.
Step 2: Electroporation – Precision Gene Editing
The art of gene editing begins here. Using the revolutionary CRISPR-Cas9 system, we introduce targeted edits to the genomic DNA of these fertilized oocytes through electroporation.
This technique is akin to opening a microscopic gateway—an electric pulse temporarily permeabilizes the oocyte membrane, allowing the CRISPR components to enter. Once inside, the CRISPR-Cas9 system acts like a molecular scalpel, precisely knocking out the target gene.
At our Institute, this method is optimized for:
Efficiency: Minimal handling ensures high survival rates.
Precision: Specific gRNAs guide the Cas9 protein to the exact genetic locus.
Step 3: Culturing Embryos – Nurturing the Future
Post-editing, the modified oocytes are transferred to a specialized culture medium. Here, under meticulously controlled conditions (37°C, 5% CO₂), they develop into robust blastocysts.
This step is a delicate balance of science and care, ensuring the embryos are primed for the next phase. It’s where the edited genetic blueprint transforms into a living, growing organism.
Step 4: Embryo Transfer – A New Home for Development
Our next step involves preparing pseudopregnant recipient mice—females mated with vasectomized males to simulate pregnancy. The cultured embryos are transferred into the uterine horns of these surrogates, where they implant and continue their development naturally.
This step is a testament to our team's expertise in embryology and animal care, ensuring the best outcomes for both the recipients and the developing embryos.
